design a protein-based assay to detect the presence of the S139N mutant protein in a patient who has been infected with Zika virus.

design a protein-based assay to detect the presence of the S139N mutant protein in a patient who has been infected with Zika virus. Briefly outline the steps of the assay, and explain how it will allow the diagnosis of the S139N Zika viral infection. (10 marks)