For the following questions, restriction enzyme digests were performed on a plasmid and the following size fragments were obtained: Sal 1:2 kb 5.1…

Q1. For the following questions, restriction enzyme digests were performed on a plasmid and the following size fragments were obtained: 

Sal 1: 0.2, 1.2 kb & 5.1 kbTuf1: 3.1kb &3.4kbSal 1, Tuf 1 double digest: 0.2, 0.3, 0.9, 2.2 and 2.9 kb       

•Station 1

  1. a)  How many total kb is the intact plasmid? 
  2. b)  How many times does Sal 1 cut the plasmid? 
  3. c)  Draw a map of the plasmid with all of the Sal 1 and Tuf 1 recognition sites clearly labeled. Indicate the relative position of the sites by labeling the distance between each site in kb.

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