You are trying to determine both the binding constant for a ligand ( L ) to protein ( P ) as well as the stoichiometry . L is available in a…

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You are trying to determine both the binding constant for a ligand ( L ) to protein ( P ) as well as thestoichiometry . L is available in a radioactive form with an activity of 10 disintegrations persecond per micromole L ( disintegration per second – DPS ) . You set up a series of equilibriumdialysis experiments and add to each side :1 micromolar pvarious quantities LDialysis membrane