Please help with this question on Enzyme Kinetics. The question is given below but do let me know if you have trouble viewing the question.
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You are trying to determine both the binding constant for a ligand ( L ) to protein ( P ) as well as thestoichiometry . L is available in a radioactive form with an activity of 10 disintegrations persecond per micromole L ( disintegration per second – DPS ) . You set up a series of equilibriumdialysis experiments and add to each side :1 micromolar pvarious quantities LDialysis membrane